Cytotoxicity analysis of Strontium Ranelate on cultured human periodontal ligament fibroblasts: a preliminary report

Er, KÜRŞAT; Akın Polat, Zübeyde; Özan, Fatih; Taşdemir, Tamer; Sezer, Ufuk; Hergüner Siso, Şeyda

doi:10.1016/s0929-6646(08)60178-3

Cytotoxicity analysis of Strontium Ranelate on cultured human periodontal ligament fibroblasts: a preliminary report

Atıf İçin Kopyala

Er K., Akın Polat Z., Özan F., Taşdemir T., Sezer U., Hergüner Siso Ş.

JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION = TAIWAN YI ZHI, cilt.107, sa.8, ss.609-615, 2008 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 107 Sayı: 8
Basım Tarihi: 2008
Doi Numarası: 10.1016/s0929-6646(08)60178-3
Dergi Adı: JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION = TAIWAN YI ZHI
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.609-615
Akdeniz Üniversitesi Adresli: Hayır

Özet

Background/purpose: The aim of this study was to analyze the cytotoxicity of strontium ranelate (SR) on human periodontal ligament fibroblasts (PDL cells) in vitro.

Methods: PDL cells were obtained from healthy human third molars and cultured in Dulbeccos Modified Eagles Medium. The experimental groups were: G1, cultures treated with fresh medium (control); and G2, G3, G4 and G5: treated with SR at 20, 10, 5 and 2.5 mg/mL, respectively. The experimental times were 1, 6, 12 and 24 hours (short-term) for viability, and 2, 4, 6 and 8 days (long-term) for cell survival. The cells were counted using a hemocytometer. Data were then analyzed by one-way ANOVA and Tukey's tests (p < 0.05).

Results: Cultures treated with the highest SR concentrations (G2 and G3) had significantly lower cell viability and cell numbers (p < 0.05) than those in G1, G4 and G5. SR at 2.5 mg/mL was non-cytotoxic to PDL cells.

Conclusion: SR was non-toxic at appropriate concentrations. Preclinical tests are needed to further assess its safety and effectiveness for tooth resorption prior to clinical use.