The investigation of the role of sirtuin-1 on embryo implantation in oxidative stress-induced mice
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, cilt.38, sa.9, ss.2349-2361, 2021 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 38 Sayı: 9
- Basım Tarihi: 2021
- Doi Numarası: 10.1007/s10815-021-02229-7
- Dergi Adı: JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, ATLA Religion Database, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
- Sayfa Sayıları: ss.2349-2361
- Anahtar Kelimeler: Implantation, Oxidative stress, Sirtuin-1, FoxO1, SOD, LIFE-SPAN, SIRT1, PARAQUAT, TRANSCRIPTION, EXPRESSION, DEACETYLASE, DETERMINES, INHIBITOR, REGULATOR, PLACENTAS
- Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
- Akdeniz Üniversitesi Adresli: Evet
Özet
Purpose Implantation is essential for a successful pregnancy. Despite the increasing number of studies, implantation is still an unknown process. This study aimed to determine whether sirtuin-1 has a role in embryo implantation in oxidative stress-induced mice. Methods Pregnant mice were separated into 5 groups: control, vehicle, paraquat, SRT1720, and SRT1720+Paraquat. Paraquat is a herbicide and is used to induce oxidative stress. SRT1720 is a specific sirtuin-1 activator. Implantation and inter-implantation sites were removed in the morning of the 5th day of pregnancy after Chicago blue injection was performed. Sirtuin-1 and Forkhead box O1 (FoxO1) were detected by immunohistochemistry and Western blot while acetylated lysine was evaluated by Western blot analysis. Reactive oxygen and nitrogen species (ROS/RNS) and superoxide dismutase (SOD) activity were determined by fluorometric and spectrometric methods, respectively. Results Although there was no embryo implantation in paraquat-treated mice, 5 out of 9 SRT1720+Paraquat-treated mice had implantation sites which were significantly higher compared to the paraquat-treated group. Sirtuin-1 and FoxO1 expressions were increased at implantation sites of SRT1720-treated mice. ROS/RNS levels were decreased, while deacetylated FoxO1 levels and SOD activity were increased in SRT1720-treated mice. Conclusion Our findings suggest that sirtuin-1 may play a role in embryo implantation against oxidative stress through FoxO1-SOD signaling.