Assessment of genetic diversity and conservation priorities among five White Leghorn Lines based on SSR markers


KARSLI T., Fidan H. G.

ANIMAL SCIENCE PAPERS AND REPORTS, vol.37, no.3, pp.311-322, 2019 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 3
  • Publication Date: 2019
  • Journal Name: ANIMAL SCIENCE PAPERS AND REPORTS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.311-322
  • Keywords: chicken lines, conservation priorities, genetic differentiation, microsatellite, CHICKEN POPULATIONS, MANAGEMENT, PROGRAM, DNA
  • Akdeniz University Affiliated: Yes

Abstract

Genetic diversity in livestock is required in breeding studies, to meet requirements of current production as well as adapt to rapidly changing environmental conditions. Identification of genetic diversity within species, breeds or lines is important for the development of conservation strategies. This study was aimed at identifying genetic diversity, population structure and conservation priorities in five white layer pure lines (Blue, Brown, D-229, Black, Maroon). In this study a total number of 150 samples (30 samples from each line) were genotyped using 19 microsatellite loci to identify genetic diversity. All loci were found to be polymorphic, with the mean number of alleles per locus ranging front 3.95 (Blue) to 4.84 (D-229). The highest average observed heterozygosity was recorded in the D-229 line (0.52), while it was lowest in the Blue line (0.41). The inbreeding coefficient ranged from 0.26 (Black) to 0.39 (Maroon). In the analyses conducted to identify conservation priorities, the chicken line with the highest contribution to total genetic diversity was found to be D-229 (1.34). Genetic differentiation coefficients (pairwise F-ST) ranged between 0.07 (Brown and D-229) and 0.20 (Blue and Black) among the chicken lines used in the study. Research findings indicate that pure chicken lines have low levels of genetic diversity, but high levels of inbreeding and genetic differentiation. Selection applied for various purposes over extensive periods of time to the chicken lines with the same genetic origin has resulted in genetic differentiation. For the sustainable use of these populations, inbreeding in lines should he reduced and genetic diversity, particularly in the D-229 line, should he conserved.