Evaluation of a CHROMagar Salmonella Medium for the Isolation of Salmonella Species


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Çekin Y., OZYURT O. K., Daglar D., DOGAN H. K., Baysan B., DALOGLU A. E., ...Daha Fazla

JOURNAL OF CLINICAL AND ANALYTICAL MEDICINE, cilt.6, sa.1, ss.29-31, 2015 (ESCI) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 6 Sayı: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.4328/jcam.1708
  • Dergi Adı: JOURNAL OF CLINICAL AND ANALYTICAL MEDICINE
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.29-31
  • Anahtar Kelimeler: Salmonella Spp, CHROMagar, Identification, STOOL SAMPLES, AGAR, ENRICHMENT, STRAINS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Aim: Salmonella infections are the leading cause of food-borne infections and can cause gastroenteritis outbreaks worldwide. Salmonella species is defined as inability to lactose fermentation, using citrate as a carbon source, using lysine as nitrate source and forming Hydrogen sulfide ((HS)-S-2) in TSI agar. However, confirmation of false positive results is time consuming and lead to increased costs. The aim of this study is to evaluate the performance of CHROMagar Salmonella (CHROMagar Microbiology, France) which is developed for isolation and detection of Salmonella species. Material and Method: For this purpose, among a total of 148 isolates which were isolated from various clinical specimens and stocked at the Central Laboratory of Akdeniz University Hospital, 65 were Salmonella spp., 10 were Pseudomonas aeruginosa, 10 were E. coli, 10 were Acinetobacter baumannii, 10 were Klebsiella pneumoniae, 18 were Morganella morganii, 11 were Citrobacter spp., 5 were Providencia spp., 4 were Aeromonas spp., 5 were Proteus spp. were included in this study. All of the 65 Salmonella spp. isolates apperared with mauve colonies at the CHROMagar Salmonella. Results: E. coli and Klebsiella pnemoniae species were seen as blue, Providencia species were seen as pale-blue; Morganella morganii species were seen as pale-pink, mauve; and Pseudomonas aeruginosa species were seen as pale. Acinebacter baumannii and Aeromonas spp. species were also seen as mauve colonies. Dicussion: CHROMagar Salmonella medium can detect Salmonella species with %100 sensitivity, however there is a need to biochemical or serological confirmation.