The Effect of Various Hormone Types on In Vitro Propagation of Strawberry


ADAK N., KAYNAK L., PEKMEZCI M., GÜBBÜK H.

6th International Symposium on In Vitro Culture and Horticultural Breeding, Brisbane, Australia, 16 June 2009, vol.829, pp.305-308 identifier

  • Publication Type: Conference Paper / Full Text
  • Volume: 829
  • City: Brisbane
  • Country: Australia
  • Page Numbers: pp.305-308
  • Keywords: tissue culture, thidiazuron, IAA, THIDIAZURON
  • Akdeniz University Affiliated: Yes

Abstract

The objective of this study was to assess the effect of Thidiazuron (TDZ) alone or in combination with IAA on both the in vitro shoot multiplication-index and plantlet quality of the strawberry cultivar 'Camarosa'. We applied the following concentrations of TDZ alone (0, 0.5, 1.0, 1.5, 2.0 mg/L) or in combination with 1.0 mg/L IAA. In addition, we applied IAA at the following concentrations: 0.4, 0.8, 1.0, 1.2 mg/L together with 1.0 mg/L TDZ. In the rooting stage, different concentrations of activated charcoal (0, 2.5, 5.0 g/L) and IBA (0, 0.1, 0.4, 0.8, 1.0 mg/L) were tested. The results demonstrated that shoot proliferation was significantly affected by TDZ alone and in combination with IAA. Our recommendation for the optimal multiplication of 'Camarosa' is: MS medium supplemented with 1.5 mg/L TDZ and 1 mg/L IAA. Using this medium we obtained 28.46 shoots per explant. As a result of this study, it was determined that the increased concentrations of IBA decreased the root development. The highest root development ratio was found in media that contains activated charcoal.