Development of resistance against broomrape (Orobanche spp., Phelipanche spp.) using CRISPR/-cas9 technology in tomato (Solanum lycopersicum)


Şimşek C., Soylu İ., Pınar H., Kaba A., Karabeniz A., Kitiş Y. E., ...Daha Fazla

2nd International Molecular Plant Protection Congress, Bursa, Türkiye, 15 - 18 Mayıs 2023, ss.52

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Bursa
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.52
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Weeds are among the most important factors that cause significant yield and quality losses in tomato (Solanum lycopersicum). Thanks to the strigolactone (SL) compounds synthesized from the tomato roots of the fully parasitic broomrape (Orobanche/Phelipanche spp.), they weaken the plant and even cause plant death by feeding from the root after germination in the soil. In this study, it was aimed to obtain genetically resistant tomato lines against broomrape with the CRISPR/Cas9 system designed to modify the “Carotenoid cleavage dioxygenase 7” (SlCCD7) gene, which is involved in the SL pathway in tomato. As a result of the transformation via the Agrobacterium-mediated method, a mutation causing a biallelic heterozygous deletion of 18 and 186 nucleotides (nt) occurred in the T0 generation. The 186 nt unique mutation resulted in an inframe modification between Thr42-Val105 in the SlCCD7-exon 1 region that resulted in the deletion of 62 amino acids. In the T1 generation, 2 of the 4 plants with 186 nt homozygous deletion were identified as marker-free (not carrying Cas9 gene). In plants carrying 186 nt homozygous deletion, broomrape resistance was obtained without undesirable agro morphological changes. Genetic resistant lines against broomrape can be used for commercial purposes in upland-greenhouse cultivation, open field industry and table tomato cultivation where solarization is not possible. It is predicted that the use of resistant varieties will greatly reduce the yield and quality losses caused by the parasitic weed.