Effect of different medium and sucrose concentrations on germination of somatic embryos in grape

Elidemir A. Y., UZUN H. İ., bayır A.

International Workshop on Advances in Grapevine and Wine Research, Venosa, İtalya, 15 - 17 Eylül 2005, ss.117-121 identifier identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Doi Numarası: 10.17660/actahortic.2007.754.15
  • Basıldığı Şehir: Venosa
  • Basıldığı Ülke: İtalya
  • Sayfa Sayıları: ss.117-121
  • Anahtar Kelimeler: Vitis vinifera, in vitro culture, culture medium, embryo stages, germination, CV CABERNET-SAUVIGNON, PLANT-REGENERATION, CULTURED ANTHERS, ABSCISIC-ACID, VITIS, EMBRYOGENESIS, CULTIVARS, INVITRO, DEHYDRATION, QUIESCENCE
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The study was aimed to obtain effect of different medium and sucrose concentrations on germination of somatic embryos of grape. Direct somatic embryogenesis from cotyledone of grape hybrid was obtained in E20A medium supplemented with 0.01 mg/L IAA. Somatic embryos were cultured in different stages (globular, heart and torpedo). They were germinated in different medium (E20A, N&N and C medium) and different sucrose concentrations (10, 30, 50 and 70 g/L). The germination rate ranged from 23% to 95% in all medium.