PROTECTION AGAINST ULTRAVIOLET B-INDUCED GENOTOXICITY BY THE CHLOROPHYLLIN IN Drosophila melanogaster

Demir E., Kocaoglu S., Kaya B.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.17, sa.12B, ss.2187-2192, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 17 Sayı: 12B
  • Basım Tarihi: 2008
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2187-2192
  • Anahtar Kelimeler: Chlorophyllin, Antigenotoxic, UVB, Drosophila melanogaster, SMART, WING SPOT-TEST, SISTER-CHROMATID EXCHANGE, SOMATIC MUTATION, RECOMBINATION TEST, GAMMA-RADIATION, SKIN, PHOTOCARCINOGENESIS, ANTIMUTAGENICITY, CARCINOGENESIS, MUTAGENICITY
  • Akdeniz Üniversitesi Adresli: Evet

Özet

In this study, the protective effect of chlorophyllin in response to UVB was investigated using the Somatic Mutation and Recombination Test in Drosophila melanogaster (SMART). UVB doses of 200, 250, 300, 350, and 400 J/cm(2) increased the frequency of all categories of spots, indicating that UVB was potentially genotoxic. Two distinct groups were formed as antimutauen pre-treatment and co-treatment. In the first group, 2-day-old Drosophila larvae were first exposed with three doses of chlorophyllin (1, 2.5, and 5 %) and then they were irradiated with the genotoxic (200, 250, 300, 350, and 400 J/cm(2)) doses of UVB at 72. In the second group, both chlorophyllin and UVB was exposed to 3-day-old Drosophila larvae simultaneously. When the results were compared with the results of individuals irradiated only genotoxic doses, it was observed that chlorophyllin have decreased genotoxicity resulted from UVB. Decreasing rate in genotoxicity was higher at 2-day-old pre-treatment groups than at 72 hours co-treatment groups The obtained results showed that three doses of chlorophyllin (1, 2.5, and 5 %) have given effective protection against UVB in Drosophila.