Sensitive sandwich-type electrochemical SARS-CoV-2 nucleocapsid protein immunosensor

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KARAMAN C., Yola B. B., KARAMAN O., Atar N., Polat I., Yola M. L.

MICROCHIMICA ACTA, cilt.188, sa.12, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 188 Sayı: 12
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1007/s00604-021-05092-6
  • Dergi Adı: MICROCHIMICA ACTA
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Pollution Abstracts, Civil Engineering Abstracts
  • Anahtar Kelimeler: COVID-19, g-C3N(4)/Au/WO3, Bi2WO6/Bi2S3, Electrochemistry, Voltammetry, Electrochemical impedance spectroscopy, Immunosensor, ENHANCED PHOTOCATALYTIC ACTIVITY, GRAPHITIC CARBON NITRIDE, PHOTOREDUCTION, DEGRADATION, POLLUTANTS, NANOSHEETS, BI2WO6, SENSOR
  • Akdeniz Üniversitesi Adresli: Evet

Özet

A sensitive and fast sandwich-type electrochemical SARS-CoV-2 (COVID-19) nucleocapsid protein immunosensor was prepared based on bismuth tungstate/bismuth sulfide composite (Bi2WO6/Bi2S3) as electrode platform and graphitic carbon nitride sheet decorated with gold nanoparticles (Au NPs) and tungsten trioxide sphere composite (g-C3N(4)/Au/WO3) as signal amplification. The electrostatic interactions between capture antibody and Bi2WO6/Bi2S3 led to immobilization of the capture nucleocapsid antibody. The detection antibody was then conjugated to g-C3N(4)/Au/WO3 via the affinity of amino-gold. After physicochemically characterization via transmission electron microscopy (TEM), scanning electron microscopy (SEM), x-ray diffraction (XRD), and x-ray photoelectron spectroscopy (XPS), cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) analysis were implemented to evaluate the electrochemical performance of the prepared immunosensor. The detection of SARS-CoV-2 nucleocapsid protein (SARS-CoV-2 NP) in a small saliva sample (100.0 mu L) took just 30 min and yielded a detection limit (LOD) of 3.00 fg m(-1), making it an effective tool for point-of-care COVID-19 testing.