EFFECTS OF TWO FUNGICIDES, CYPRODINIL AND FLUDIOXONIL, ON GENOTOXICITY IN DROSOPHILA SMART ASSAY AND ON PROLIFERATION AND VIABILITY OF HEK293 CELLS FROM THE PERSPECTIVE OF CARCINOGENESIS

Karadeniz A., KAYA B., Savaş B., TOPCUOĞLU Ş. F.

FRESENIUS ENVIRONMENTAL BULLETIN, cilt.24, sa.5B, ss.1920-1925, 2015 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 5B
  • Basım Tarihi: 2015
  • Dergi Adı: FRESENIUS ENVIRONMENTAL BULLETIN
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED)
  • Sayfa Sayıları: ss.1920-1925
  • Anahtar Kelimeler: Drosophila melanogaster, wing spot test, cyprodinil, fludioxonil, genotoxic effect, cancer proliferation, WING SPOT-TEST, RECOMBINATION TEST, SOMATIC MUTATION
  • Akdeniz Üniversitesi Adresli: Evet

Özet

In this study, the mutagenic and recombinogenic effects of fungicides; cyprodinil and fludioxonil, widely used in agricultural regions, were investigated using the somatic mutation and recombination test (SMART) in Drosophila wings. The viability and proliferation effects also tested on a human immortalized embryonic kidney HEK293 cells which is at the early stage of carcinogenesis were also examined with MTT and trypan-blue exclusion assays. For the SMART assay, two different crosses were used: a standard (ST) and a high-bioactivation (HB) cross, involving the flare-3 (flr(3)) and the multiple wing hairs (mwh) markers. The HB cross involved flies characterized by an increased cytochrome P-450-dependent bioactivation capacity, which permits the more efficient biotransformation of promutagens and procarcinogens. In both crosses, the wings of the two types of progeny, inversion-free marker heterozygotes and balancer heterozygotes, were analyzed. The results show that both fungicides were recombinogenic at the tested highest concentration, on the wing cells of Drosophila. Furthermore, neither fungicide affected the proliferation rate of the HEK293 cells but both induced cell death at high concentrations.