Akademik Veri Yönetim Sistemi
V. International Plant Breeding Congress, Antalya, Türkiye, 1 - 05 Aralık 2025, cilt.1, ss.3, (Özet Bildiri)
Bacterial canker of tomato, caused by the Gram-positive bacterium Clavibacter michiganensis subsp. michiganensis (Cmm), represents one of the most devastating diseases affecting global tomato production. This seed-borne pathogen has caused catastrophic epidemics in major tomato-growing regions worldwide. A prominent control is Cmm-resistant cultivars for tomato production is a suitable way for sustainable and eco-friendly method. For genetic control of the Cmm, a chemical mutagenized susceptible EBR3 tomato lines generated resistant M3-9 and M3-15 plants. Aims of the study are to identify resistance gene(s) controlling Cmm pathogen, to understand resistant gene(s) mediated mechanisms and to examine differences in gene expression between resistant mutants and susceptible original EBR3 plants. High-throughput sequencing was performed on an Illumina HiSeq platform for these three genotypes. After the filtering process, each individual FASTQ file was aligned to the Solanum lycopersicum reference genome “SLM_r2.1” with using Bowtie2 software. They were properly mapped with the values of >99.8 for all genotypes. Genotype specific individual binary sequence alignment file format (BAM) files were subjected to variant calling using freebayes. All variants from these genotypes were merged. Potential gene regions were monitored with the use Integrated Genome Browser and compared with disease scores obtained from EBR3, M3-9, and M3-15 plants. A total of 17 potential regions related to resistance to canker and wilting disease were identified.