A comparative study on protoplast isolation protocol from different explants of strawberry
Acta Horticulturae, cilt.1465, ss.173-178, 2026 (Scopus)
- Yayın Türü: Makale / Özet
- Cilt numarası: 1465
- Basım Tarihi: 2026
- Doi Numarası: 10.17660/actahortic.2026.1465.28
- Dergi Adı: Acta Horticulturae
- Derginin Tarandığı İndeksler: Scopus
- Sayfa Sayıları: ss.173-178
- Anahtar Kelimeler: callus, explant, Fragaria × ananassa, protoplast isolation, protoplast viability
- Akdeniz Üniversitesi Adresli: Evet
Özet
This study explored the efficient isolation of protoplasts from various strawberry explants, including leaf mesophyll and leaf callus. A comparative analysis of protoplast yield and viability was conducted across these tissue types. Leaves were harvested from in vitro-grown strawberry plants for mesophyll protoplasts. In contrast, callus tissues were cultured on three different media: T1 (MS + 1 mg L-1 BAP, 0.1 mg L-1 IBA, 3% sucrose, 0.7% agar, pH 5.7), T2 (MS + 2 mg L-1 2,4-D, 0.5 mg L-1 kinetin, 3% sucrose, 0.7% agar, pH 5.7), and T3 (MS + 2 mg L-1 NAA, 3% sucrose, 0.7% agar, pH 5.7). Various concentrations of mannitol, enzymes, infiltration times, and incubation periods were evaluated to optimize protoplast isolation. The highest callus formation of all explants was observed in the T3 medium. The results demonstrated the successful isolation of protoplasts from all explants, with potential implications for further strawberry breeding and genetic research applications.