Investigation of Apoptotic Potential of Catechol in Lung Cancer and Healthy Fibroblast Cells


Özkan A., Demir Z.

MolBiyoKon'22 8. International Congress of the Molecular Biology Association of Turkey, İstanbul, Türkiye, 9 - 12 Haziran 2022, ss.139

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: İstanbul
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.139
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Abstract

Background/aim: Many herbal compounds are applied for phytotherapy purposes in lung

cancer (H1299), the incidence of which is increasing globally. However, it is a fact that this

compound, which will be used to treat lung cancer, also affects healthy cells. Activation of the

apoptotic pathway was investigated to demonstrate the anticancer effect of catechol on lung

cancer cells. In addition, the activation of the apoptotic pathway was studied with healthy

fibroblast (Bj) cells and the results were compared.

Materials and methods: The 24-hour cytotoxic effect of catechol on cells was demonstrated

by the Cell Titer-Blue® cell viability test. In order to determine the apoptotic potential, the

caspase 3/7 activity of the cells exposed to catechol was measured and the potential of

catechol to stimulate the apoptotic pathway was determined. Caspase 3/7 enzyme activity was

determined using the Promega 'ApoTox-GloTM Triplex Assay kit.

Results: In our study, the IC50 values of the cytotoxic effect of catechol on lung cancer and

healthy fibroblast cells were found to be 58 and 207 μg/ml, respectively. Caspase-3/7 activity

in lung cancer and Bj cells increased 2.8-fold and 1.6-fold, respectively, compared to control

after 24 hours of catechol incubation.

Conclusion: In conclusion, lung cancer cells have higher caspase 3/7 activity than healthy cells,

indicating that catechol has a higher apoptotic potential in cancer cells than in healthy cells.

Keywords: Catechol, Lung cancer, Apoptosis

Acknowledgment : The authors wish to thank Akdeniz University, BAP-Scientific Research

Projects Coordination Unit (FYL-2022-5902) for financial support of this work.