Standard aggregating media to test the "aggregability" of rat red blood cells

Baskurt O., Bor-Kucukatay M., Yalcin O., Meiselman H., Armstrong J.

CLINICAL HEMORHEOLOGY AND MICROCIRCULATION, vol.22, no.2, pp.161-166, 2000 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 22 Issue: 2
  • Publication Date: 2000
  • Journal Name: CLINICAL HEMORHEOLOGY AND MICROCIRCULATION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.161-166
  • Keywords: erythrocyte aggregation, aggregability, standard aggregating media, rat, ERYTHROCYTE AGGREGATION
  • Akdeniz University Affiliated: No

Abstract

Rat red blood cells (RBC) exhibit low aggregation tendency in autologous plasma and in standard aggregating media (e.g., 3% Dextran 70; MW: 70 kD). In experimental studies performed on rats, 3% Dextran 70 was found to be an unsuitable suspending medium to test the "aggregability" of RBC in a standard medium. It has been observed that solutions of higher molecular weight polymers (i.e., dextran, MW: 500 kD; polyethylene glycole, MW: 35 kD; polyvinylpyrrolidone, MW: 360 kD), at low concentrations were strong aggregators for rat RBC. Among these polymer solutions 0.5% Dextran 500 and 0.75% polyvinylpyrrolidone 360 were found to be the most suitable suspending media, to distinguish between the aggregability of RBC from septic and control rats. Therefore, these two polymer solutions are recommended as the standard aggregating media for rat RBC, to test the RBC aggregability.