Molecular identification of Meloidogyne luci using KASP assay

DEVRAN Z., Göknur A.

Physiological and Molecular Plant Pathology, cilt.140, 2025 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 140
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.pmpp.2025.102888
  • Dergi Adı: Physiological and Molecular Plant Pathology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, BIOSIS, CAB Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Detection, Identification, KASP, M. luci
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Meloidogyne luci is one of the most important root-knot nematodes (RKNs) attacking crops in many parts of the world. It was identified as an emerging nematode species and included in the EPPO Alert List in 2017. Rapid and accurate identification of M. luci is required for management practices and laboratory applications. In the present study, a high-throughput Kompetitive Allele-Specific PCR (KASP) assay was developed for molecular identification of M. luci. The assay easily separated M. luci from the closely related species and other RKN species. This is the first report on the identification of M. luci using a KASP assay. The KASP primers designed for this study can be used for rapid and accurate identification of M. luci.