Nitrate Reductase Assay for Rapid Determination of Methicillin-Resistant Staphylococcus aureus Clinical Isolates

Yildirim K., Atas C., Tanyel Akcit E., Licina M., Koyuncu Ozyurt Ö., Gun M. A., ...Daha Fazla

Lab Medicine, cilt.55, sa.2, ss.174-178, 2024 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 55 Sayı: 2
  • Basım Tarihi: 2024
  • Doi Numarası: 10.1093/labmed/lmad056
  • Dergi Adı: Lab Medicine
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, CINAHL
  • Sayfa Sayıları: ss.174-178
  • Anahtar Kelimeler: colorimetric method, infectious disease, methicillin-resistance, nitrate reductase assay, rapid detection, Staphylococcus aureus
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Objective: To evaluate the performance of nitrate reductase assay (NRA), a rapid, colorimetric method for the determination of methicillin resistance in Staphylococcus aureus isolates obtained from the culture collection of the Akdeniz University Hospital Central Laboratory, Antalya, Türkiye. Materials and Methods: Identification for all 290 S aureus isolates at the species level was performed via matrix-assisted laser desorption/ionization-time of flight. Isolates were tested with NRA for methicillin resistance. The cefoxitin broth microdilution (BMD) method recommended by the Clinical and Laboratory Standards Institute was used as the reference method in the study. S aureus ATCC 29213 and S aureus ATCC 43300 strains were used for quality control. Results: According to Food and Drug Administration criteria, the category agreement between NRA and BMD was found to be 100%. The essential agreement between both methods was determined to be 96.20%. There is no minor, major, or extremely major discrepancy between both methods. Conclusion: The results show that NRA is a rapid, practical, and reliable colorimetric method for detecting MRSA.