Absence of human herpesvirus-8 in pulmonary inflammatory myofibroblastic tumor: immunohistochemical and molecular analysis of 20 cases


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TAVORA F., SHILO K., Ozbudak I. H., PRZYBOCKI J. M., WANG G., TRAVIS W. D., ...Daha Fazla

MODERN PATHOLOGY, cilt.20, sa.9, ss.995-999, 2007 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 20 Sayı: 9
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1038/modpathol.3800938
  • Dergi Adı: MODERN PATHOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.995-999
  • Anahtar Kelimeler: inflammatory myofibroblastic tumor (IMT), human herpesvirus-8 (HHV-8), lung, EPSTEIN-BARR-VIRUS, FIBROBLAST CELLS, LYMPHOMA-KINASE, DNA-SEQUENCES, PSEUDOTUMOR, INFECTION, DISEASE, 2P23, GENE, AMPLIFICATION
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Inflammatory myofibroblastic tumors are uncommon lesions composed of spindled myofibroblasts within a variable background of collagen and inflammatory cells. Although the true nature of these lesions is not fully elucidated, identification of consistent cytogenetic alterations in the anaplastic lymphoma kinase ( ALK) gene suggests that they may be neoplastic. A small number of inflammatory myofibroblastic tumors have been reported to harbor human herpesvirus-8 ( HHV-8), implicating the virus in its pathogenesis. In this study, 20 cases of pulmonary inflammatory myofibroblastic tumor were analyzed for the presence of HHV-8 with immunohistochemical and molecular methods. In all cases, antibodies to the latent nuclear antigen of the virus were applied. Four open reading frames ( ORFs), including ORFs K2, 16, 26, and 72, were targeted utilizing realtime polymerase chain reaction ( PCR). The cohort included 9 men and 11 women with a mean age of 37 years ( range, 1-81). Microscopically, the tumors were composed of cytologically bland spindle cells with myofibroblastic differentiation. On immunohistochemical studies, 20% of cases ( 4/ 20) demonstrated diffuse cytoplasmic positivity with ALK. Immunohistochemical staining for the latent nuclear protein of the virus was negative in all cases ( 0/20). All tumors ( 100%, 20/20) tested with real-time PCR were negative for all four ORFs, whereas 100% ( 10/10) of positive control Kaposi sarcoma cases were positive. Her2 gene expression was present in all ( 20/ 20) inflammatory myofibroblastic tumors confirming the presence of amplifiable deoxyribonucleic acid in the tissue lysate. This study documents the absence of HHV-8 in pulmonary inflammatory myofibroblastic tumors, suggesting that further investigation is required to clarify the pathogenesis of this lesion.