The purpose of this research is to determine the effect of web-based instruction with educational animation content at sensory organs subject on the 7th grade students' academic achievement and attitudes in the science and technology course. Quasi-experimental research method was applied in this study. For this purpose, as a means of measuring, Science and Technology achievement test consisting of 30 questions and 15-itemed Science Course attitude scale developed by Geban et. al. (1994) were used. In experimental group, the sensory organs were explained with web-based instructional materials with educational animation content, and academic achievement test and attitude scale were applied as post-test; in the control group, subject of sensory organs were rendered in the traditional lecture method and application is finished with applying academic achievement test and attitude scale as post-test. As consequence of study, there is a meaningful difference in favor of experimental group on the academic achievement pre-test / post-test marks between experimental group instructed with web-based instructional materials with educational animation content and control group instructed with traditional lecture method. It is found that there is no significant difference on attitudes towards science and technology. (C) 2013 The Authors. Published by Elsevier Ltd.