Development of molecular markers for the Mi-1 gene in tomato using the KASP genotyping assay

DEVRAN Z., GOKNUR A., MESCI L.

HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY, vol.57, no.2, pp.156-160, 2016 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 2
  • Publication Date: 2016
  • Doi Number: 10.1007/s13580-016-0028-6
  • Journal Name: HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.156-160
  • Keywords: FRET, resistance gene, root-knot nematodes, sequence, SNP, NEMATODE RESISTANCE GENE, ROOT-KNOT NEMATODE, LEAF CURL VIRUS, LOCUS
  • Akdeniz University Affiliated: Yes

Abstract

Tomato (Solanum lycopersicum) is an economically important vegetable worldwide. Root-knot nematodes have a wide host range and cause serious problems in areas where tomatoes are grown. Developing tomato varieties that are resistant to root-knot nematodes is an effective crop management strategy. The Mi-1 gene in tomato confers effective resistance against three common root-knot nematodes, i.e., Meloidogyne incognita, M. javanica, and M. arenaria. Nematode screening assays are commonly used in resistance breeding programs; however, they are time-consuming, laborious, and expensive. Molecular markers within, or linked to, the Mi-1 gene have enabled rapid screening of resistance alleles, without requiring nematode inoculation. Here we describe convenient markers for the Mi-1 gene locus using Kompetitive Allele-Specific PCR (KASP) genotyping chemistry. Sequences of SNP markers for resistance to root-knot nematodes on chromosome 6 were converted for usage in a KASP genotyping assay and tested. Our findings show that the KASP assay is suitable for high-throughput screening of large populations in tomato breeding.