ULUSAL TRAVMA VE ACIL CERRAHI DERGISI, cilt.29, sa.11, ss.1228-1236, 2023 (SCI-Expanded)
Background: This study aims to investigate whether the
expression levels of proteins involved in microRNA (miRNA) biogenesis vary in
the early- and late-stage traumatic brain injury (TBI) patients and to evaluate
its effect on prognosis.
Methods: Dicer, Drosha, DiGeorge Syndrome Critical Region 8
(DGCR8), Exportin5 (XPO5), and Argonaute2 (AGO2) levels were measured in the
blood samples of severe TBI patients collected 4 to 6 hours and 72 hours after
the trauma and compared with the control group. Prognostic follow-up of the
patients was performed using the Glasgow Coma Scale score.
Results: There were no statistically significant changes in
the expression of the miRNA biogenesis proteins Dicer, Drosha, DGCR8, XPO5, and
AGO2 in patients with severe TBI. However, the expression of Dicer increased in
the patients who improved from the severe TBI grade to the mild TBI grade, and
the expression of AGO2 decreased in most of these patients. The Dicer
expression profile was found to increase in patients discharged from the
intensive care unit in a short time.
Conclusion: MicroRNAs and their biogenesis proteins may
guide prognostic and therapeutic decisions for patients with TBI in the future.
Keywords: Traumatic brain injury, Prognosis, microRNA, microRNA
biogenesis