Measurement of erythrocyte deformability and methodological adaptation for small-animal microsurgical models


Nemeth N., Gulyas A., Balint A., Peto K., Brath E., Kiss F., ...Daha Fazla

MICROSURGERY, cilt.26, sa.1, ss.33-37, 2006 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 26 Sayı: 1
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1002/micr.20207
  • Dergi Adı: MICROSURGERY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.33-37
  • Akdeniz Üniversitesi Adresli: Hayır

Özet

Measuring erythrocyte deformability with bulk filtrometers is common, but there are problems with standardization and sample quantity in small laboratory animals. In this study, blood was drawn from mice, rats, and dogs, and then erythrocyte suspensions at 1 - 5% hematocrit were prepared. Bulk filtrometers with a 5- or 3-mu m pore-diameter filter were used to determine the initial relative filtration rate (IRFR) and relative cell transit time (RCTT). Hematological composition was also tested. In all species, the highest IRFR values occurred at 1% hematocrit, and the lowest values at 5%. In mice and rats, RCTT values were lowest at 1% and highest at 5% using a 5-mu m filter, whereas in dogs, RCTT was elevated at 1 - 2%. Using a 3-mu m filter, each species showed the same phenomena under 3%. RCTT values may become distorted above a certain cell-size/pore-size ratio. In mice and rats, 1% suspension is applicable for bulk filtrometry, but only using 5-mu m filters. (c) 2006 Wiley-Liss, Inc.