Effect of chronic L-carnitine supplementation on carnitine levels, oxidative stress and apoptotic markers in peripheral organs of adult Wistar rats.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, cilt.134, ss.110851, 2019 (SCI-Expanded)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 134
- Basım Tarihi: 2019
- Doi Numarası: 10.1016/j.fct.2019.110851
- Dergi Adı: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.110851
- Anahtar Kelimeler: L-carnitine, Apoptosis, Oxidative stress, ACETYL-L-CARNITINE, BODY-COMPOSITION, WEIGHT-LOSS, INJURY, METABOLISM, ANTIOXIDANT, PROTECTS, FATIGUE, PATHWAY
- Akdeniz Üniversitesi Adresli: Evet
Özet
This study investigated the effects of L-carnitine supplementation on carnitine levels, oxidative stress and apoptotic markers in the stomach, kidney, liver and testis tissues in adult rats. Rats were randomized to control and L-carnitine supplemented (LCAR) groups. Control group received distilled water for 7 months by intragastric gavage and the LCAR group was given 50 mg/kg/day L-carnitine via intragastric intubation for the same period. L-carnitine concentrations and caspase-3 activity were measured by fluorometric methods while cleaved caspase-3 was determined by Western blot analysis. Bcl-2 associated X protein (Sax) and B-cell lymphoma/leukemia-2 (Bcl-2) were quantified by enzyme immunoassay and Western blot analysis. Oxygen/nitrogen species (ROS/RNS) and total antioxidant capacity (TAC) were analyzed by colorimetric assay. Tissue L-carnitine concentrations were significantly increased in the LCAR group compared to controls. Anti-apoptotic Bcl-2 levels were significantly increased while pro-apoptotic Bax was significantly decreased in LCAR group rats compared to controls. Tissue caspase-3 was significantly alleviated in the LCAR group compared to controls. L-carnitine supplementation increased TAC and decreased ROS/RNS generation in the kidney, liver, stomach and testis tissues compared to controls. Obtained data suggests that L-camitine supplementation can potentially be used to lessen both oxidative and apoptotic progression in peripheral organs.