The effects of type I collagen on bone defects and gene expression changes for osteogenesis: In a rat model

Yigiter O., YÖRÜKOĞLU A. Ç., Sentuerk N., DODURGA Y., DEMİRKAN A. F.

JOURNAL OF CELLULAR BIOCHEMISTRY, cilt.120, sa.7, ss.11525-11530, 2019 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 120 Sayı: 7
  • Basım Tarihi: 2019
  • Doi Numarası: 10.1002/jcb.28432
  • Dergi Adı: JOURNAL OF CELLULAR BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.11525-11530
  • Anahtar Kelimeler: bone defects, bone morphogenic protein, gene expression, osteogenesis, type I collagen, REGENERATION, MATRIX, REPAIR, FRACTURE, CELLS, OSTEOBLASTS, IMPLANTS, ADHESION, PROTEIN, GROWTH
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The aim of this study is to investigate the effects of type I collagen on bone defects and on genes specifically for osteogenesis in a rat model. Two millimeter drill hole bone defect was created in the femur of rats. In the experimental group, type I collagen was applied in bone defects whereas in control group defects were left empty. Inflammation, development of connective tissue, osteogenesis, and foreign body reaction parameters evaluated with histologically and genes evaluated by blood samples. In the experimental group, the histopathologically significant change was found in favor of bone healing only at the first week. A significant increase was found in genetic expressions of BMP-1, 2, 3, 4, 5, 6, 7, TGF-RII, Smad-1, IL-6, BMPR-IA, BMPR-IB, Eng, BMPR-II, c-fos, Cdkn1a, Chrd, Gdf-5, Id-1, PDGF-, IGF-1, Serpine-1, and TGF-RI at the first hour. At the first, third, and sixth week, no significant increase was found in any of the gene expressions. Type I collagen is found to be effective in favor of bone healing through increased inflammatory cytokines and expression of BMP genes in the early stages of fracture healing.