Akademik Veri Yönetim Sistemi
JOURNAL OF CLINICAL AND ANALYTICAL MEDICINE, cilt.6, sa.3, ss.309-311, 2015 (ESCI)
Aim: Acinetobacter species are amoung the most common two cause of infections isolated from patients of intensive care unit in our hospital. Doripenem which acts by inhibiting cell wall synthesis is resently introduced for use in our country is broad spectrum antibiotic belonging to carbapenems. There are many studies investigating the susceptibility of doripenem of Acinetobacter baumannii which is isolated as a cause of ventilatory associated pneumonia in the literature. We aimed to compare e-test and disc diffusion methods for doripenem susceptibility of acinetobacter baumannii strains as nosocomial infections Acinetobacter baumanni isolates detected as nosocomial infection. Material and Method:. Between January to December, 2009 a total of 94 Acinetobacter baumanni strains isolated from different clinical specimens from intensive care units have been studied for doripenem susceptibility by disc diffusion and E-test methods. Minimal inhibitory consantrations (MIC) were accepted as; sensitive <= 1 mu g/ml, intermadiate 2-4 mu g/ml, resistant > 4 mu g/ml and diameters of inhibition zone with 10 mu g disc; sensitive < 15mm, resistant >= 21mm according to European Committee on Antimicrobial Susceptibility Testing (EUCAST). Results: In our study, 10 isolates were sensitive, 17 isolates were intermediate, 67 isolates were resistant by disc diffision method whereas 12 isolates were sensitive, 12 isolates were intermediate and 70 isolates were resistant by E-test method. The categorical aggreement between disc diffusion and E-test methods (intermedite isolates were considered as resistant) were found as 88.3% (83/94). In our study major disrepancy and minor discrepancy rates were 1.2% (1/2) and 18.1% (17/94) respectively. There was no very major discrepancy. Discussion: As a result in routine laboratory practice there is a high level of aggreement of determining doripenem susceptibility of Acinetobacter spp by disc diffusion and E-test mehods.