Akademik Veri Yönetim Sistemi
VIII. International Seed Congress, Antalya, Türkiye, 9 - 12 Aralık 2024, cilt.1, ss.1
The bacterial wilt and canker disease caused by Clavibacter michiganensis subsp. michiganensis (Cmm) is seed transmitted especially spreading throughout all tomato (Solanum lycopersicum) grown places, the bacteria are resulting serious losses to both greenhouse and field tomato crops either killing whole plant or reducing tomato yields. A prominent control is Cmm-resistant cultivars for tomato production is a suitable way for sustainable and eco-friendly method. The mutant lines were generated by using 0.5% Ethylmethanesulfonate (EMS) chemical mutagen from susceptible NCEBR3 plant; M3-9 and M3-15 plants were determined as resistant against Cmm isolate 2 at M3 population previously. Individual mapping populations were constructed with M3-9 and M3-15 mutant line and a resistance locus was mapped at chromosome 5 of tomato. The resistance locus was linked with SL20210_883i SSR marker flanked by single nucleotide polymorphism markers. For understanding and cloning genetic polymorphisms, entire 5th chromosomes were sequenced to reveal Cmm-resistance locus using Illumina HiSeq. There were 20 mutations were investigated between M3-9 and M3-15 mutant plants, and 53 mutations among susceptible NCEBR3, M3-9 and M3-15 mutant plants. Preliminary results showed that there were 8 uncharacterized proteins and 11 serine/threonine-protein phosphatase and 2 acylsugar acyltransferase 3-like proteins found between 2 mutant lines. Further, next generation sequencing analyses will precisely exhibit which proteins are governing resistance to Cmm2 bacteria.