7'th International Congress on Adhesive Dentistry (IAD), Konya, Türkiye, 1 - 03 Haziran 2023, ss.44
Introduction: The aim of this study is to evaluate the long-term bonding performance of existing universal adhesives to
sound (SD) and caries-affected (CAD) dentin using microtensile bond strength test, electron microscopy (SEM), and
confocal laser scanning microscopy (CSLM) images taken from bond interfaces.
Materisals and Methods: A total of 120 sound and decayed human molars were ground to a flat surface by lowering the
dentin level with a precision cutting device until the soft caries lesions reached the hard dentin layer. The surface
microhardness was determined by Vickers microhardness test by applying 25 g load for 15 seconds and divided into 3
groups (sound dentin-SD, two levels of caries-affected dentin-D1 and D2). Dentin samples were divided into 4 subgroups;
Group 1: Peak Universal Bond (PUB)+ Self-Etch (SE), Group 2: PUB+Etch and Rinse (ER), Group 3: G-Premio Bond (GPB)+SE, Group 4: G-PB+ER. A nanohybrid universal resin composite structure was prepared on the specimens. After
soaking the specimens in 37 C0
distilled water for 24 hours and 1 year, the microtensile bond strength (μTBS) test and
fracture type analysis, resin-dentin interface analysis by SEM and CSLM were performed. One-way analysis of variance
(One-way ANOVA), Scheffe multiple comparison test and independent t-test were used for statistical analysis and the
significance level was set at p≤ 0.05.
Results: After 24 hours, the SD group at PUB-SE recorded the highest bond strength value (36.80 MPa), and the D1 group
at G-PB SE recorded the lowest value. (17.60 MPa). After a year, the SD group at G-PB SE recorded the highest bond
strength value (30.38 MPa), while the D1 group at PUB SE recorded the lowest value. (16.67 MPa).
Conclusion: It was found that the Vickers test was a useful tool for differentiating between SD and CAD, that the bond
strength of universal adhesives to CAD was significantly lower than SD, and that there was a significant difference between
the various modes of application.
Keywords: caries-affected dentin, microhardness, universal adhesive, microtensile bond strength, confocal laser microscopy