Selective disruption of "late onset" sagittal banding patterns by ectopic expression of Engrailed-2 in cerebellar Purkinje cells

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BAADER S., VOGEL M., Sanlioglu S., ZHANG X., OBERDICK J.

JOURNAL OF NEUROSCIENCE, vol.19, no.13, pp.5370-5379, 1999 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 13
  • Publication Date: 1999
  • Doi Number: 10.1523/jneurosci.19-13-05370.1999
  • Journal Name: JOURNAL OF NEUROSCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.5370-5379
  • Keywords: mouse, cerebellum, Purkinje cell, Engrailed-2, mossy fibers, pcp-2(L7), cadherin, zebrin, NOS, OXIDE SYNTHASE EXPRESSION, ZEBRIN-II, PARASAGITTAL ORGANIZATION, REVEALS COMPARTMENTS, GENE-EXPRESSION, GRANULE CELLS, MESSENGER-RNA, OPTIC TECTUM, MOSSY FIBERS, MUTANT MICE
  • Akdeniz University Affiliated: No

Abstract

To explore the role of Engrailed proteins in development of the cerebellum, Engrailed-2 (En-2) was ectopically expressed in cerebellar Purkinje cells from the late embryonic stage into adulthood. The fundamental organization of Purkinje cell sagittal zones as revealed by the "early onset" markers L7-beta-gal and cadherin-8 was found to be virtually identical to that in wild type. In contrast, "late onset" sagittal banding patterns revealed by Purkinje cell markers zebrin I, zebrin II, and 9-O-acetyl G(D3) Ganglioside (P-Path), and the granule cell marker NADPH-diaphorase, were disrupted. In general, although some evidence of banding was still detectable, boundaries defined by the latter markers were poorly defined, and the patterns overall took on a diffuse appearance. In parallel with the changes in late onset markers, anterograde tracing of spinocerebellar axons revealed a general diffusion of the mossy fiber projection pattern in lobule VIII and the anterior lobe, These observations suggest that at least two separate mediolateral boundary systems exist in the cerebellum, and these are differentially affected by ectopic En-2 expression. Alternatively, one boundary system exists that remains primarily intact in the mutant, but recognition of this system by a set of late developmental events is perturbed.