Akademik Veri Yönetim Sistemi
WASPaLM World Congress and XXIV. National Clinical Biochemistry Congress , Antalya, Türkiye, 16 - 20 Ekim 2024, (Yayınlanmadı)
Aim: Celiac disease, triggered by gluten exposure in genetically susceptible individuals, is a chronic
autoimmune condition. While small intestine biopsy remains the diagnostic 'gold standard,' serological tests like
tissue transglutaminase antibodies have gained importance. However, their high cost and limited availability
present challenges.Inflammatory cytokines play a role in celiac disease, with monocytes expressing them
notably. Some studies suggest increased activation of circulating monocytes in celiac patients, possibly linked to
intestinal epithelial damage. Vitamin D, beyond its classical role, regulates cell differentiation and proliferation,
exhibiting anti-inflammatory effects. Research indicates its potential in reducing inflammatory factors secreted
by monocytes, possibly protecting against inflammation. Studying complete blood count parameters for
systemic inflammation and disease activity has become increasingly common due to easy affordability and
accessibility. The Systemic Inflammatory Response Index evaluates overall inflammatory response. This study
aims to evaluate Systemic Inflammatory Response Index and vitamin D in Celiac disease.
Methods: The study included 46 patients recently diagnosed with Celiac disease (25 girls and 21 boys; mean
age: 10.89±4.40 years) and 52 healthy controls (28 girls and 24 boys; mean age: 10.96±3.02). The complete
blood count was performed using the Sysmex XN-1000 automated hematology analyzer (Sysmex Inc, Kobe,
Japan). Systemic Inflammatory Response Index was calculated by (neutrophil count×monocyte
count)/lymphocyte count. Vitamin-D levels were detected by electrochemiluminescence immunoassay on
Roche Elecsys system.
Results: The total leukocyte counts (x1000/uL) were 7.90 ± 2.58 and 6.96±1,62 (p=0.03), the neutrophil counts
were 4.29 ± 2.34 and 3.44 ± 1.19 (p=0.02), the lymphocyte counts were 2,78 ± 0,96 and 2,61 ± 0,85 (p >0.05)
and monocyte counts were 0.59 ± 0.21, and 0.43 ± 00.14 (p<0.0001) in patient group and control group,
respectively. Systemic Inflammatory Response Index was determined as 1,19 ± 1,31 in the celiac group and
0.62 ± 0.34 in the control group (p=0.003). When evaluating the diagnostic significance of monocyte levels and
Systemic Inflammatory Response Index measurements, the area under the ROC curve were calculated as 0.73
and 0.63, respectively. Although the vitamin D level (μg/L) was lower in the patient group than control group,
there was no significant difference between the groups (20.61 ± 7.92 and 21.61 ± 6.89 (p>0.05). There was a
negative correlation between vitamin D levels and % monocyte counts (r:-0.270, p=0.07). However, this
relationship was not statistically significant.
Discussion: In our study, the systemic inflammatory response index and monocyte values were found to be
significantly higher in the celiac patient group. With this finding, considered that these parameters could be used
for the diagnosis of celiac disease in situations where tissue transglutaminase-IgA cannot be measured. In the
celiac group, a negative correlation was observed between vitamin D and monocyte percentage; however, this
correlation was not found to be statistically significant. Nevertheless, it is thought that re-evaluating this
relationship in studies with increased sample sizes would be beneficial.