Endostatin and irradiation modifies the activity of ADAM10 and neprilysin in breast cancer cells

Creative Commons License

Aydemir E., ŞİMŞEK E., Korcum A. F., FIŞKIN K.

MOLECULAR MEDICINE REPORTS, cilt.14, sa.3, ss.2343-2351, 2016 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 3
  • Basım Tarihi: 2016
  • Doi Numarası: 10.3892/mmr.2016.5463
  • Dergi Adı: MOLECULAR MEDICINE REPORTS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2343-2351
  • Anahtar Kelimeler: ADAM10, Co-60 irradiation, endostatin, neprilysin, substance P, NEUTRAL ENDOPEPTIDASE-24.11 NEPRILYSIN, HUMAN HEPATOCELLULAR CARCINOMAS, NK1 RECEPTOR ANTAGONISTS, LINES IN-VITRO, SUBSTANCE-P, LUNG-CANCER, ANGIOGENESIS INHIBITORS, ANTIANGIOGENIC ACTIVITY, HEART-FAILURE, TUMOR-GROWTH
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Angiogenesis, the formation of new blood vessels, is regarded as a key cancer cell property. Endostatin (ES) is a potential antiangiogenic agent and it may be useful when implemented in combination with other cancer therapeutic strategies. The present study investigated the in vitro effects of ES, radiotherapy (RT) or combination therapy (ES + RT) on two important proteases, a disintegrin and metalloproteinase domain-containing protein 10 (ADAM10) and neprilysin (NEP) in 4T1 mouse breast cancer cells and the more metastatic phenotype of 4THMpc breast cancer cells. 4T1 and 4THMpc cells were treated with recombinant murine ES (4 g/ml) alone, RT (45 Gy) alone or with ES + RT. ADAM10 enzyme activity was determined using a tumor necrosis factor- converting enzyme (-secretase) activity assay kit, and NEP enzyme activity was measured with a fluorometric assay based on the generation of free dansyl-D-Ala-Gly from N-dansyl-Ala-Gly-D-nitro-Phe-Gly, the substrate of NEP. Western blotting analysis was performed to determine whether the altered enzyme activity levels of the two cell lines occurred due to changes in expression level. These data indicate that ES independently potentiates the activity of ADAM10 and NEP enzymes in 4T1 and 4THMpc breast cancer cells.