Boron containing nano hydroxy apatites (B-N-HAp) Stimulate mesenchymal stem cell adhesion, proliferation and differentiation

Ciftci E., KÖSE S., KORKUSUZ P., Timuçin M., KORKUSUZ F.

26th Symposium and Annual Meeting of the International Society for Ceramics in Medicine, ISCM 2014, Barcelona, İspanya, 6 - 08 Kasım 2014, cilt.631, ss.373-378 identifier

  • Yayın Türü: Bildiri / Tam Metin Bildiri
  • Cilt numarası: 631
  • Basıldığı Şehir: Barcelona
  • Basıldığı Ülke: İspanya
  • Sayfa Sayıları: ss.373-378
  • Anahtar Kelimeler: Bioceramics, Boron, Hydroxyapatite, Nano, Stem cell
  • Akdeniz Üniversitesi Adresli: Evet

Özet

© (2015) Trans Tech Publications, Switzerland.Osteoporosis (OP) is a systemic metabolic disease identified with decrease of bone mineral density and deterioration of microstructure leading to fragility fractures in elderly. Boron (B) is assumed to stimulate osteoblasts. Hydroxyapatite (HAp) is clinically used to conduct bone regeneration and improves implant integration. Nano(n)-HAp expands the surface area for cell adhesion and may improve bone regeneration and tissue integration. The objective of this study was to examine the adhesion, proliferation and differentiation of B-n-HAp with mesenchymal stem cells (MSC's). Human bone marrow derived MSC's phenotype was assessed using scanning and transmission electron microscopy after combining with B-n-HAp and n-HAp. Cell adhesion and proliferation potential of these ceramics was examined with the real time cell analysis (xCELLigence, Roche Applied Science and ACEA Bioscience, USA) system and adipogenic-osteogenic differentiation was analyzed with morphological and quantitative methods. MSC's adhesion and proliferation rates (cell index, 4.50) were higher than controls (cell index, 4.00). Adipogenic and osteogenic differentiation potential of MSC's remained unchanged in the presence of B-n-HAp ceramics. In conclusion, B-n-HAp stimulates MSC's adhesion, proliferation and differentiation and has a potential to regenerate bone tissue.