A practical, low-cost, short-term storage method for genomic DNA
BIOTECHNIQUES, vol.70, no.4, pp.194-201, 2021 (SCI-Expanded)
- Publication Type: Article / Article
- Volume: 70 Issue: 4
- Publication Date: 2021
- Doi Number: 10.2144/btn-2020-0158
- Journal Name: BIOTECHNIQUES
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Communication Abstracts, EMBASE, MEDLINE, Metadex, Veterinary Science Database, Directory of Open Access Journals, DIALNET, Civil Engineering Abstracts
- Page Numbers: pp.194-201
- Keywords: cellulose, DNA preservation, dried blood spots, FTA, paper towel
- Akdeniz University Affiliated: Yes
Abstract
The aim of this study was to assess the DNA preservation capability of cellulose paper towel and blotting paper as low-cost alternatives to commercial DNA preservation products. Chicken blood was applied as DNA source to each paper towel, blotting paper, FTA(R) cards and DNA/RNA Shield (TM). All samples were stored at room temperature for 130 days. DNA extraction from dried blood spots was performed after various time periods using Tris-EDTA and NaOH protocols. PCR activity and the mean amount of DNA isolated from paper towels were reliable. The results of this study demonstrated that cellulose-based blotting paper and especially paper towel had considerable DNA binding and preservation capacity for at least 130 days at room temperature without DNA degradation.