Alterations in Growth Related Genes (GH-I, IGF-I and IGF-II) Expression with Acute Copper Exposure in Rainbow Trout

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Aksakal E., CEYHUN S. B., Beydemir S., ERDOĞAN O.

JOURNAL OF ANIMAL AND VETERINARY ADVANCES, vol.10, no.25, pp.3334-3339, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 10 Issue: 25
  • Publication Date: 2011
  • Doi Number: 10.3923/javaa.2011.3334.3339
  • Journal Name: JOURNAL OF ANIMAL AND VETERINARY ADVANCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.3334-3339
  • Keywords: Copper, growth hormone, insulin like growth factors, gene expression, multiplex real-time PCR, rainbow trout, MOLECULAR-CLONING, ZEBRAFISH, FISH, METALLOTHIONEIN, HORMONE, PATTERNS, STRESS, FORMS, LIVER, MODEL
  • Open Archive Collection: AVESIS Open Access Collection
  • Akdeniz University Affiliated: No

Abstract

In this study, the acute effects of copper (CuSO4 center dot 5H(2)O) on the expression of Growth Hormone-I (GH-I) and Insulin like Growth Factors I and II (IGF-I and IGF-II) were investigated in rainbow trout (Oncorhynchus mykiss) muscle tissue. For this purpose, rainbow trout were exposed to 100 mu g L-1 (Sublethal concentration) copper sulfate in moderately hard freshwater. After 6, 12, 24 and 48 h of treatment, expression of white muscle GH-I, IGF-I and IGF-II mRNAs were measured by means of quantitative real-time PCR. During the exposure experiments, no mortalities occurred. All mRNA levels significantly decreased after copper administration (p<0.05). The results indicate that short term copper exposure suppresses the mRNA expression levels of GH-I, IGF-I and IGF-II in rainbow trout muscles.