NeutralizingBDNFandFGF2injection into denervated skeletal muscle improve recovery after nerve repair

Rink S., Chatziparaskeva C., Elles L., Pavlov S., Nohroudi K., Bendella H., ...Daha Fazla

MUSCLE & NERVE, cilt.62, sa.3, ss.404-412, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 62 Sayı: 3
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1002/mus.26991
  • Dergi Adı: MUSCLE & NERVE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CINAHL, EMBASE, MEDLINE
  • Sayfa Sayıları: ss.404-412
  • Anahtar Kelimeler: axotomy, BDNF, FGF2, functional recovery, motoneuron, motor endplates, polyinnervation, vibrissae whisking, FIBROBLAST-GROWTH-FACTOR, TERMINAL SCHWANN-CELLS, IN-VIVO OBSERVATIONS, NEUROTROPHIC FACTOR, AXONAL REGENERATION, POSTSYNAPTIC CHANGES, FUNCTIONAL RECOVERY, VIBRISSAL MOVEMENT, TROPHIC FACTORS, RAT
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Background After facial nerve injury and surgical repair in rats, recovery of vibrissal whisking is associated with a high proportion of mono-innervated neuro-muscular junctions (NMJs). Our earlier work with Sprague Dawley (SD)/Royal College of Surgeons (RCS) rats, which are blind and spontaneously restore NMJ-monoinnervation and whisking, showed correlations between functional recovery and increase of fibroblast growth factor-2 (FGF2) and brain-derived neurotrophic factor (BDNF) in denervated vibrissal muscles. Methods We used normally sighted rats (Wistar), in which NMJ-polyinnervation is highly correlated with poor whisking recovery, and injected the vibrissal muscle levator labii superioris (LLS) with combinations of BDNF, anti-BDNF, and FGF2 at different postoperative periods after facial nerve injury. Results Rats receiving anti-BDNF+FGF2 showed low NMJ-polyinnervation and best recovery of whisking amplitude. Conclusions Restoration of target reinnervation after peripheral nerve injury requires a complex mixture of trophic factors with a specific time course of availability for each of them.