Characterization of Pseudomonas tolaasii Isolates, Causing Brown Blotch Disease of Mushroom (Agaricus bisporus)

BASIM E., BASIM H.

INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY, vol.20, no.12, pp.2721-2730, 2018 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 12
  • Publication Date: 2018
  • Doi Number: 10.17957/ijab/15.0819
  • Journal Name: INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.2721-2730
  • Keywords: Agaricus bisporus, Brown blotch disease, Conventional PCR, Pseudomonas tolaasii, Real-time PCR, BACTERIAL DISEASE, WHITE-LINE, IDENTIFICATION
  • Akdeniz University Affiliated: Yes

Abstract

A total of 96 Pseudomonas tolaasii isolates were recovered from the suspected Brown blotch infected mushroom caps and cultured on King's medium B. The LOPAT test results showed Levan and pectinolytic activity, positive oxidase, arginine dihydrolase and hypersensitivity on tobacco. The isolates was identified by the conventional PCR using Pt-1A/Pt-1D1 primers and Real-time PCR. The BLAST analysis of the PCR products showed 98-100% similarities with the strains recovered from the NCBI GenBank database. All the isolates were pathogenic on Agaricus bisporus and slices of potatoes. The Koch's postulates were fulfilled. This is the first record on the characterization of P. tolaasii isolates in west Mediterranean region of Turkey as well as rapid detection of this pathogen directly from the bacterial cell without DNA extraction and from the diseased tissue, respectively by the Real-time PCR. (C) 2018 Friends Science Publishers