Magnetic-Activated Cell Sorting of Human Spermatozoa


DİRİCAN E. K.

in: Practical Manual of In Vitro Fertilization: Advanced Methods and Novel Devices, Nagy ZP, Varghese AC, Agarwal A, Editor, Springer, New York, pp.265-272, 2012

  • Publication Type: Book Chapter / Chapter Research Book
  • Publication Date: 2012
  • Publisher: Springer
  • City: New York
  • Page Numbers: pp.265-272
  • Editors: Nagy ZP, Varghese AC, Agarwal A, Editor
  • Akdeniz University Affiliated: Yes

Abstract

Magnetic cell sorting allows large numbers of viable cells to be isolated with high purity and yield. It is accepted that the process does not cause physical damage to the cell. Today, magnetic nanoparticles are widely used in separation of macromolecules or cells from various cell suspensions. Studies have shown that nonapoptotic sperm fractions selected by MACS technique have morphologically superior quality, higher percentage of motility, viability, improved apoptosis indices and routine sperm parameters. Reduction of apoptotic spermatozoa within the ejaculate by means of the MACS system results in a distinct reduction of spermatozoa with DNA fragmentation. The initial clinical trials of MACS of human spermatozoa in assisted reproduction showed a statistically significant improvement in the embryo cleavage rates.

A number of studies, including our own, have shown that using spermatozoa prepared with MACS technique significantly improves the quality of spermatozoa in the preparation. The efficiency of this new technique is still not fully evaluated, and the interpretation of ICSI results still remains incomplete. More research is needed to improve our current knowledge in relation to human sperm apoptosis and MACS technique.