The effects of Eicosapentaenoic acid on the endothelium of the carotid artery of rabbits on a high-cholesterol diet

Cayli S., Sati L., Seval-Celik Y., Tuncer M. A., Yaymaci B., Berkman Z., ...Daha Fazla

HISTOLOGY AND HISTOPATHOLOGY, cilt.25, sa.2, ss.141-151, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 25 Sayı: 2
  • Basım Tarihi: 2010
  • Dergi Adı: HISTOLOGY AND HISTOPATHOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.141-151
  • Anahtar Kelimeler: EPA, Cholesterol, Atherosclerosis, Endothelium, Ultrastructure, Rabbit, ALL-CIS-5,8,11,14,17-ICOSAPENTAENOATE EPA-E, POLYUNSATURATED FATTY-ACIDS, FISH-OIL SUPPLEMENTATION, BLOOD-PRESSURE, ETHYL ALL-CIS-5,8,11,14,17-ICOSAPENTAENOATE, PLASMA-LIPIDS, DOCOSAHEXAENOIC ACID, LIPOPROTEINS, VASCULOGENESIS, ANGIOGENESIS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

The preventive and therapeutic effects of Eicosapentaenoic acid (EPA) on diet-induced hyperlipidemia in rabbits have been investigated. Eighteen New Zealand rabbits were randomly divided into three groups of 6 subjects each; experimental group-I (EG-I) was administered a cholesterol rich diet, experimental group-II (EG-II) was treated with EPA (300 mg/kg/d) following a cholesterol-rich diet and the control group (CG) had a standard diet. Blood samples were collected at day 0 and at the 4th and 12th weeks of EG-II to obtain serum levels of total cholesterol (TC), high density lipid-cholesterol (HDL-C), low density lipid-cholesterol (LDL-C) and triglyceride (TG). From each group tissue samples were collected from the carotid artery for immunohistochemistry and electron microscopy. Our results showed that EPA could significantly lower (p<0.001) serum TC, LDL-C, HDLC and TG levels with a reduction of 35%; 55%; 44% and 51%, respectively. Scanning and transmission electron microscopy results revealed that endothelial damage was more prominent in EG-I when compared to EG-II. The ruptured endothelial lining and damaged cellular surface was increased in EG-I when compared to EG-II. Ultrastructural observations showed that after EPA treatment, the degeneration and cellular surface damage on the endothelium were also decreased.