Next day determination of ejaculatory sperm motility after overnight shipment of semen to remote locations

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Sati L., Bennett D., Janes M., Huszar G.

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, cilt.32, sa.1, ss.117-125, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 1
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1007/s10815-014-0365-2
  • Dergi Adı: JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.117-125
  • Anahtar Kelimeler: Sperm mitochondrial activity, MitoTracker, Sperm velocity, Shipped semen, TRIS-CITRIC ACID, HUMAN SPERMATOZOA, FLOW-CYTOMETRY, OXIDATIVE STRESS, CELL VIABILITY, EGG-YOLK, MITOCHONDRIA, FERTILITY, STORAGE, EXTENDER
  • Akdeniz Üniversitesi Adresli: Evet

Özet

To develop a method for delayed assessment of sperm motility, after shipment of semen to a remote laboratory. Sperm in semen were labeled with the MitoTrackerA (R) Red CM-H(2)XRos reagent, and fixed with 3.7 % formaldehyde by the laboratory technicians at the origin of the semen. This treatment reflected well sperm mitochondrial activity, and the MitoTrackerA (R) signal was related to sperm motility and velocity for 2-3 days following ejaculation.