The molecular patterns of resistance to anti-tuberculosis drugs: an analysis from Istanbul, Turkey

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Yazısız H., Cenger D. H., Ucarman N., Altin S.

JOURNAL OF CHEMOTHERAPY, cilt.32, sa.2, ss.66-74, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 2
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/1120009x.2020.1716477
  • Dergi Adı: JOURNAL OF CHEMOTHERAPY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, EMBASE, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.66-74
  • Anahtar Kelimeler: Genotype MTBDRplus, Genotype MTBDRsl, Mycobacterium tuberculosis, drug resistance, MDR-TB, XDR-TB, GENOTYPE(R) MTBDRPLUS ASSAY, MYCOBACTERIUM-TUBERCULOSIS, DIAGNOSTIC PERFORMANCE, MTBDRSL ASSAYS, SUSCEPTIBILITY, ETHAMBUTOL, FLUOROQUINOLONES, SURVEILLANCE, MECHANISMS, MUTATIONS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Resistances to anti-tuberculosis (TB) drugs are related to the mutations in the genome of the Mycobacterium tuberculosis complex (MTBC). To expose the genomic mutations that cause anti-TB drug resistance. The GenoType MTBDRplus and MTBDRsl assays were used to detect genetic mutations. In this study of 1329 MTBC isolates, the sensitivities and specificities of genotypic methods for the detection of isoniazid (INH), rifampicin (RIF), ethambutol (EMB), and multi-drug resistance were 0.77, 0.84, 0.65, 0.89 and 0.99, 0.98, 0.67, 0.94, respectively. MUT3 mutations (S531L) of the rpoB gene for RIF resistance and MUT1 mutations (S315T1 and C15T) of the katG and inhA genes for INH resistance were dominant. The most frequently observed mutations that created resistance to fluoroquinolones (FLQ) were MUT3C (D94G) of the gyrA gene. The predominant mutations of EMB resistance were MUT1B (M306V) of the embB gene. Aminoglycosides/cyclic peptides (AG/CP) resistance was rare. The molecular mechanisms of anti-TB drugs resistance in MTBC strains found in Istanbul are similar to those in the literature.