Monitoring of BLAD, DUMPS, CVM, BC and FXID in Turkish Native Cattle Breeds

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Meydan H., Ugurlu M., YILDIZ M. A.

JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI, cilt.18, sa.3, ss.239-245, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Sayı: 3
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1501/tarimbil_0000001211
  • Dergi Adı: JOURNAL OF AGRICULTURAL SCIENCES-TARIM BILIMLERI DERGISI
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.239-245
  • Anahtar Kelimeler: BLAD, DUMPS, CVM, BC, FXID, PCR-RFLP, FACTOR-XI DEFICIENCY, URIDINE MONOPHOSPHATE SYNTHASE, COMPLEX VERTEBRAL MALFORMATION, LEUKOCYTE ADHESION DEFICIENCY, BOVINE CITRULLINEMIA, MOLECULAR DEFINITION, HOLSTEIN CATTLE, IDENTIFICATION, PREVALENCE, CARRIERS
  • Akdeniz Üniversitesi Adresli: Hayır

Özet

In this study, Turkish native cattle breeds were monitored with respect to the genetic disorders defined as bovine leukocyte adhesion deficiency (BLAD), deficiency of uridine monophosphate synthase (DUMPS), complex vertebral malformation (CVM), bovine citrullinaemia (BC) and factor XI deficiency (FXID). All these are autosomal recessive hereditary disorders causing serious economic losses in dairy cattle breeding throughout the world. In order to determine the presence or the absence of BLAD, DUMPS, CVM, BC and FXID genotypes in native cattle breeds, 200 heads of Anatolian Black, 100 heads of East Anatolian Red, 100 heads of Turkish Grey, 50 heads of Anatolian Southern Yellow, 50 heads of South Anatolian Red and 9 heads of Zavot breed (totally 509 heads) were sampled. Genomic DNA was obtained from blood and the amplicons were obtained by using PCR. PCR products were digested with TaqI, AvaI, EcoT22I and AvaII restriction enzymes for BLAD, DUMPS, CVM, and BC, respectively. Digested products of BLAD, DUMPS CVM and BC were analyzed by agarose gel electrophoresis. PCR products of FXID were analyzed by only agarose gel electrophoresis stained with ethidium bromide. The results demonstrated that no animals examined were the carrier of these genetic disorders. Thus, it can be concluded that mutant alleles of BLAD, DUMPS, CVM, BC and FXID are absent in Turkish native cattle breeds.

In this study, Turkish native cattle breeds were monitored with respect to the genetic disorders defined as bovine leukocyte
adhesion deficiency (BLAD), deficiency of uridine monophosphate synthase (DUMPS), complex vertebral malformation
(CVM), bovine citrullinaemia (BC) and factor XI deficiency (FXID). All these are autosomal recessive hereditary disorders
causing serious economic losses in dairy cattle breeding throughout the world. In order to determine the presence or the
absence of BLAD, DUMPS, CVM, BC and FXID genotypes in native cattle breeds, 200 heads of Anatolian Black, 100 heads
of East Anatolian Red, 100 heads of Turkish Grey, 50 heads of Anatolian Southern Yellow, 50 heads of South Anatolian Red
and 9 heads of Zavot breed (totally 509 heads) were sampled. Genomic DNA was obtained from blood and the amplicons
were obtained by using PCR. PCR products were digested with TaqI, AvaI, EcoT22I and AvaII restriction enzymes for
BLAD, DUMPS, CVM, and BC, respectively. Digested products of BLAD, DUMPS CVM and BC were analyzed by
agarose gel electrophoresis. PCR products of FXID were analyzed by only agarose gel electrophoresis stained with ethidium
bromide. The results demonstrated that no animals examined were the carrier of these genetic disorders. Thus, it can be
concluded that mutant alleles of BLAD, DUMPS, CVM, BC and FXID are absent in Turkish native cattle breeds.
Keywords: BLAD; DUMPS; CVM; BC; FXID; PCR-RFLP