Plasma levels of nitrites, PGF(1 alpha) and nitrotyrosine in LPS-treated rats: functional and histochemical implications in aorta

Ozturk O. H., Cetin A., Ozdem S. S., Uysal N. E., Kayisli U. A., Senturk U. K., ...Daha Fazla

JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY, cilt.62, sa.1, ss.27-34, 2006 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 62 Sayı: 1
  • Basım Tarihi: 2006
  • Dergi Adı: JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.27-34
  • Anahtar Kelimeler: lipopolysaccharide, nitrites, NO, prostacyclin, nitrotyrosine, aorta, OXIDE SYNTHASE, PROSTACYCLIN SYNTHASE, ENDOTOXIC-SHOCK, IN-VIVO, PEROXYNITRITE, CYCLOOXYGENASE, ENDOTHELIUM, ACTIVATION, LIPOPOLYSACCHARIDE, INDUCTION
  • Akdeniz Üniversitesi Adresli: Evet

Özet

We investigated the effects of lipopolysaccharlde (LPS) administration on plasma nitrite, nitrotyrosine and 6-keto prostaglandin F-1 alpha (PGF(1 alpha)) levels and the related resultant changes in function and histochemistry of aorta in rats. Plasma nitrite and PGF(1 alpha), nitrotyrosine levels were analysed after 5 mg/kg intravenous LPS was administered to rats compared with those in non-treated rats. The distribution of nitrotyrosine in the aorta was studied immunohistochemically. The contractile responses of aortic rings to phenylephrine (PE) from both the LPS-treated and control rats were studied in the organ baths. There were increases in plasma nitrite, PGF(1 alpha), and nitrotyrosine concentrations of LPS-treated rats compared to non-treated rats. Immunoreactivit), of nitrotyrosine residues were detected in the endothelial and smooth muscle cells in LPS-treated but not in control rat aorta. The contractile responses to PE of the LPS-treated rat aortic rings were significantly reduced as compared with those of control rat's. Incubation of the aortic rings from LPS-treated rats with cyclooxygenase inhibitor indomethacine or with a combination of indomethacine and nitric oxide synthase inhibitor N-omega-nitro-L-arginine methyl ester (L-NAME) increased the contractile responses to the levels observed in control rats suggesting that both prostanoids and particularly nitric oxide (NO) are involved in the reduced contractile responses in LPS-treated rats. These results supported the view that LPS might cause an increment in both NO and PGI(2) levels. This increase in the NO and PGI(2) levels may be responsible from the reduction in responses of aorta to contractile agents in LPS-treated rats. Increased peroxynitrite formation in LPS-treated rats may lead to nitration of the tyrosil residues of the proteins in the aorta.