Akademik Veri Yönetim Sistemi
III. International Eurasian Agriculture and Natural Sciences Congress, Antalya, Türkiye, 17 - 19 Ekim 2019
The secretory pathway has an essential role in the localization of proteins to correct subcellular compartments in all eukaryotes. An initial step in cargo sorting is the budding of COPII coated vesicles from the ER and their trafficking to the cis Golgi network in a process called anterograde transport. The inner layer of the COPII coat contains Sar1 GTPase, SEC23 and SEC24 proteins. While Chlamydomonas is an excellent model organism for cell biology, it has not been used for functional analyses of components of COPII vesicles. Chlamydomonas has paralogs of both the SEC23 and SEC24 genes. Our objective is to determine the functional differences among these parologs. We plan to use CRISPR technology to generate mutations in each paralog. In addition, we have recently begun to characterize insertional mutant lines in each of these SEC genes; phenotypic analyses of these mutants will provide insights into the functions of each of them in the trafficking of proteins to their sites of function.