The value of fluorimetry (Qubit) and spectrophotometry (NanoDrop) in the quantification of cell-free DNA (cfDNA) in malignant melanoma and prostate cancer patients.

Ponti, Giovanni; Maccaferri, Monia; Manfredini, Marco; Kaleci, Shaniko; Mandrioli, Mauro; Pellacani, Giovanni; Ozben, TOMRİS; Depenni, Roberta; Bianchi, Giampaolo; Pirola, Giacomo; Tomasi, Aldo

doi:10.1016/j.cca.2018.01.007

The value of fluorimetry (Qubit) and spectrophotometry (NanoDrop) in the quantification of cell-free DNA (cfDNA) in malignant melanoma and prostate cancer patients.

Copy For Citation

Ponti G., Maccaferri M., Manfredini M., Kaleci S., Mandrioli M., Pellacani G., ...More

Clinica chimica acta; international journal of clinical chemistry, vol.479, pp.14-19, 2018 (SCI-Expanded)

Publication Type: Article / Article
Volume: 479
Publication Date: 2018
Doi Number: 10.1016/j.cca.2018.01.007
Journal Name: Clinica chimica acta; international journal of clinical chemistry
Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Page Numbers: pp.14-19
Keywords: Cell-free fDNA, NanoDrop, Spectrophotometry, Fluorimetry, Qubit, qPCR, Malignant melanoma, Prostate cancer, Laboratory biomarkers, Circulating free tumor DNA, Tumor marker, BLOOD
Akdeniz University Affiliated: Yes

Abstract

Background: Circulating cell-free tumor DNA (cfDNA) is of crucial interest in oncology. cfDNA constitutes a potential prognostic and therapeutic marker for different solid tumors and can be used in the diagnostic and therapeutic management of cancer patients for which nowadays there are no valid laboratory markers. In the present study, the quality and quantity of the cfDNA were assessed by different quantification procedures, in order to identify the potential applications of these techniques in the preliminary cfDNA quantification.