HIGH-FREQUENCY TRANSFORMATION FROM CULTURED COTYLEDONS OF ARABIDOPSIS-THALIANA ECOTYPES C24 AND LANDSBERG ERECTA

BARGHCHI M., TURGUT K., SCOTT R., DRAPER J.

PLANT GROWTH REGULATION, vol.14, no.1, pp.61-67, 1994 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 14 Issue: 1
  • Publication Date: 1994
  • Doi Number: 10.1007/bf00024142
  • Journal Name: PLANT GROWTH REGULATION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.61-67
  • Keywords: ARABIDOPSIS, REGENERATION, COTYLEDON, TRANSGENIC PLANTS, ANTIBIOTICS, PLANT-REGENERATION, AGROBACTERIUM-TUMEFACIENS, MEDIATED TRANSFORMATION, INVITRO MORPHOGENESIS, TISSUE-CULTURES, EXPLANTS
  • Akdeniz University Affiliated: No

Abstract

Procedures were developed for rapid and prolific adventitious shoot regeneration of Arabidopsis thaliana (L.) Heynh ecotypes ''Landsberg erect'' and ''C24'' from cotyledon explants at 90-100% efficiency. Immature cotyledons had the highest shoot regeneration efficiency. Prolific regeneration was achieved in Murashige and Skoog medium (MS) supplemented with 0.1-0.4 mg(-1) naphthalene acetic acid (NAA) and 1.0 mg(-1) 6-benzylaminopurine (BAP) within 2 weeks. The regenerated plants had a normal phenotype and produced fertile flowers and set seeds. The above regeneration protocol was used to develop a transformation method using disarmed strains of Agrobacterium tumefaciens strains pGV3850::pH1121 based on kanamycin (Km) selection. Transgenic shoots were produced within 2-3 weeks after inoculation. Transformation of shoots was confirmed by GUS histochemical assay, as well as southern blot hybridization.