Assessment of Cytotoxicity Activity of Cyclamen graecum and Prospero autumnale Using Brine Shrimp (Artemia salina L., 1758) Lethality Bioassay


Yalım F. B., Mammadov R., Emre Y., Düşen S., Düşen O., EMRE N.

2nd International Symposium on Limnology and Freshwater Fisheries, Elazığ, Turkey, 3 - 05 September 2019, vol.1, no.1, pp.137

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Elazığ
  • Country: Turkey
  • Page Numbers: pp.137
  • Akdeniz University Affiliated: Yes

Abstract

Various extracts of different plant species are known to have cytotoxic, antifungal,
antimicrobiological, anticancer, antioxsidant effects, but, it is very important to know the toxic effects of these products used as additives for the safe use.
Cytotoxic activities of two geophyte species, Cyclamen graecum and Prospero autumnale were detected by brine shrimp (Artemia salina L., 1758) lethality bioassay in this study.
The extracts were obtained from below-ground-above-ground of Cyclamen graecum and
Prospero autumnale. Brine shrimp eggs were hatched in 500 ml artificial sea water prepared from sea salt under artificial light at 28 ?C. After 48 h, nauplii specimens.were collected with a pasteur pipette and were added to each set of tubes containing 0.5 ml plant extracts and filled to 5 mL total volume with artificial salt water.
The test extracts prepared including control and with three different concentrations (10 ppm, 100 ppm, and 1000 ppm) . After 24 h, the number of survivors was counted and the percentage of death calculated. LC50 values were calcuted by Probit Analysis Program. We found that above-ground of P. autumnale with 131.27 ppm exhibited higher cytotoxic activity than C. graceum with 321.42 ppm.
Keywords: Cytotoxicity, Brine shrimp, lethality bioassay, Cyclamen graecum, Prospero autumnale