The most intensive cultivation and breeding studies conducted in the genus Anemone has been actualized in Anemone coronaria L. species. However, the inbreeding process takes at least 8-10 years because of high level of heterozygosity in the species. The trouble in the obtaining uniform pure lines has oriented the breeders towards the haploidization techniques. In few haploidization studies performed in A.coronaria so far, anther culture technique was preferred. But, it could not be found detailed literature about the morphology of suitable flower bud to be used in androgenesis studies. In the study, it was aimed to determine the developmental stages of microspores in the flower buds of wild A.coronaria var. coccinea and to describe the morphology of the suitable bud having uninucleate microspores. For this purpose, the flower buds in different size were separated into 10 groups. Different cytologic and histological dying techniques were used for identification of the microspores’ developmental stages. According to the findings, the flower buds in the second and third group containing the microspores in the stages of late-tetrad and uninucleate were determined to be the most suitable buds for androgenesis studies of A.coronaria var. coccinea. The morphological markers of them have been described as: Young buds stands curved especially when they first appears on the soil surface and creates a quite narrow angle almost parallel with the pedicels. They are approximately 6x9 mm in size and their tepals do not seen from the outside since the involucrum leaves are closed. Additionally, it was determined that the microspores in an anther are homogeneously developed while the anthers within a bud show asynchronous development.