Turkish Journal of Agriculture and Forestry, cilt.50, sa.1, ss.80-88, 2026 (SCI-Expanded, Scopus, TRDizin)
In Slovakia, snowy mespilus ( Amelanchier × lamarckii F. G. Schroed.) is recognized both as an ornamental plant and a lesser-known fruit species, prized for its high levels of bioactive compounds, particularly polyphenols. The aim of this study was to detect the polyphenolic profile of snowy mespilus and to assess polyphenol accumulation in cells. The total polyphenol content was determined by the Folin–Ciocalteu method, antioxidant activity by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay and the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) method, and individual polyphenolic compounds by the high- performance liquid chromatography ( HPLC) method. The results of analyses revealed that among the phenolic acids, chlorogenic acid was the most prevalent [44.24 ± 0.2 μg/g fresh weight (FW); 123.75 μg/g ± 1.0 dry weight (DW)], while among the flavonoids, epigallocatechin (13.17 ± 0.2 μg/g FW; 36.84 ± 0.3 μg/g DW) and quercetin (7.6 ± 0.0 μg/g FW; 21.25 ± 0.1 μg/g DW) were dominant. These polyphenolic compounds are primarily accumulated in the epidermis and hypodermis cells of the fruits, with anthocyanin granules being the predominant polyphenols.