Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Jacobs, Pieter; Inan, MEHMET; Festjens, Nele; Haustraete, Jurgen; Van Hecke, Annelies; Contreras, Roland; Meagher, Michael; Callewaert, Nico

doi:10.1186/1475-2859-9-93

Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Jacobs P. P., Inan M., Festjens N., Haustraete J., Van Hecke A., Contreras R., ...Daha Fazla

MICROBIAL CELL FACTORIES, cilt.9, 2010 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 9
Basım Tarihi: 2010
Doi Numarası: 10.1186/1475-2859-9-93
Dergi Adı: MICROBIAL CELL FACTORIES
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Açık Arşiv Koleksiyonu: AVESİS Açık Erişim Koleksiyonu
Akdeniz Üniversitesi Adresli: Evet

Özet

Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce.