Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Jacobs, Pieter; Inan, MEHMET; Festjens, Nele; Haustraete, Jurgen; Van Hecke, Annelies; Contreras, Roland; Meagher, Michael; Callewaert, Nico

doi:10.1186/1475-2859-9-93

Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

Jacobs P. P., Inan M., Festjens N., Haustraete J., Van Hecke A., Contreras R., ...More

MICROBIAL CELL FACTORIES, vol.9, 2010 (SCI-Expanded, Scopus)

Publication Type: Article / Article
Volume: 9
Publication Date: 2010
Doi Number: 10.1186/1475-2859-9-93
Journal Name: MICROBIAL CELL FACTORIES
Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Open Archive Collection: AVESIS Open Access Collection
Akdeniz University Affiliated: Yes

Abstract

Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce.