Akademik Veri Yönetim Sistemi
ACTA HISTOCHEMICA, cilt.107, sa.3, ss.173-185, 2005 (SCI-Expanded)
The reorganization of the human endometrium is termed decidualization, which includes endometrial. cell proliferation, differentiation, integrin switching and extracellular matrix (ECM) remodeling during early pregnancy. The present study aimed to investigate distribution patterns, staining intensity and sex steroid-mediated regulation of integrin alpha 5 (CD49e), integrin beta 4 (CD49f) expression and their ligands fibronectin and laminin during decidualization. Human tissue samples were evaluated in two groups, those collected in early days and those collected in advanced days of the first trimester. Correlating immunostaining was found between laminin and integrin beta 4, and between fibronectin and integrin alpha 5. The expression of fibronectin was higher than that of Laminin in the early days (p<0.05). Temporal and spatial immunostaining of integrin beta 4 and alpha 5 in the apical pole of luminal and glandular cells was observed as pregnancy progressed (p<0.05). In vitro results showed that human chorionic gonadotropin (hCG) stimulated laminin expression, downregulated integrin beta 4 expression, whereas estradiol. decreased fibronectin expression by Ishikawa cells. hCG suppressed fibronectin expression in endometrial. stromal cells in culture. Our results suggest that fibronectin is responsible for induction of decidual. cell differentiation, and different temporal and spatial expression of the integrins may play a role in implantation. Our in vitro results suggest that regulation of extracellular matrix remodeling and integrin switching are at least partially regulated by reproductive hormones. (c) 2005 Elsevier GmbH. All rights reserved.