Immunohistochemistry of Paraffin Sections from Mouse Ovaries.

Akkoyunlu G., Tepekoy F.

Methods in molecular biology (Clifton, N.J.), cilt.1457, ss.269-74, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 1457
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1007/978-1-4939-3795-0_20
  • Dergi Adı: Methods in molecular biology (Clifton, N.J.)
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.269-74
  • Anahtar Kelimeler: Immunohistochemistry, Mouse ovary, Paraffin sections, Bouin's Fixative, Biotinylated antibodies, ANTIBODY, BIOTIN, TUMORS
  • Akdeniz Üniversitesi Adresli: Evet

Özet

Immunohistochemistry (IHC) is an efficient technique to detect cellular localizations of the proteins in paraffin-embedded tissues. It allows specific proteins to be visualized by the interaction of antibodies with an enzyme-substrate-chromogen system. Here, we describe indirect immunohistochemistry method for paraffin-embedded mouse ovaries fixed with Bouin's Fixative.