Immunohistochemistry of Paraffin Sections from Mouse Ovaries.
Methods in molecular biology (Clifton, N.J.), vol.1457, pp.269-74, 2016 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 1457
- Publication Date: 2016
- Doi Number: 10.1007/978-1-4939-3795-0_20
- Journal Name: Methods in molecular biology (Clifton, N.J.)
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.269-74
- Keywords: Immunohistochemistry, Mouse ovary, Paraffin sections, Bouin's Fixative, Biotinylated antibodies, ANTIBODY, BIOTIN, TUMORS
- Akdeniz University Affiliated: Yes
Abstract
Immunohistochemistry (IHC) is an efficient technique to detect cellular localizations of the proteins in paraffin-embedded tissues. It allows specific proteins to be visualized by the interaction of antibodies with an enzyme-substrate-chromogen system. Here, we describe indirect immunohistochemistry method for paraffin-embedded mouse ovaries fixed with Bouin's Fixative.