Optimization of Salmonella bacteriophage microencapsulation in alginate-caseinate formulation using vibrational nozzle technique

ERGİN F., Atamer Z., ÇOMAK GÖÇER E. M., DEMİR M., Hinrichs J., KÜÇÜKÇETİN A.

FOOD HYDROCOLLOIDS, cilt.113, 2021 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 113
  • Basım Tarihi: 2021
  • Doi Numarası: 10.1016/j.foodhyd.2020.106456
  • Dergi Adı: FOOD HYDROCOLLOIDS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Food Science & Technology Abstracts, INSPEC, Veterinary Science Database
  • Anahtar Kelimeler: Felix O1, Salmonella enterica, Microencapsulation, Simulated gastric fluids, Simulated intestinal fluid
  • Akdeniz Üniversitesi Adresli: Evet

Özet

In this study, phage Felix O1 which has ability to infect Salmonella enterica, was microencapsulated by using extrusion method with sodium alginate and sodium caseinate. The formation of microcapsules with the highest microencapsulation efficiency, the highest resistance to low pH and the smallest perimeter was investigated by optimization of process variables applied in bacteriophage microencapsulation. Response Surface Methodology was used for the optimization of the encapsulation process. The optimum conditions were obtained by changing the ratio of sodium alginate content to sodium caseinate content of microencapsulating mixture and the vibration frequency during extrusion process. The optimum conditions for the highest microencapsulation efficiency (OptME) value predicted by the model were: vibration frequency value of 1.61 kHz, the sodium alginate content of 1.86% and sodium caseinate content of 3.26% in the microencapsulating mixture. The lowest reduction rate of microencapsulated phage Felix O1 titers (OptR) was achieved at the frequency value of 4.50 kHz, and with using the microencapsulating mixture contained 2.00% of sodium alginate and 2.69% of sodium caseinate. The activity of microencapsulated phage Felix O1 produced under optimized conditions in the simulated gastric and intestinal fluids was determined. The lowest decrease in the phage titers of Felix O1 was detected in the microcapsules produced for OptR, in the simulated gastric fluid. All microcapsules produced under optimum conditions were successfully released in simulated intestinal fluid. In conclusion, the optimum conditions obtained by the Response Surface Methodology can be used to phage Felix O1 microencapsulation in solutions of sodium alginate and sodium caseinate with extrusion method.